Serum-Free Recombinant Production of Adenovirus Using a Hollow Fiber Capillary System

Serum-Free Recombinant Production of Adenovirus Using a Hollow Fiber Capillary System

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A novel method for the production of adenoviral vectors on moen finney a scale sufficient to support most research applications and early phase clinical trials is presented.This method utilizes serum-free cell culture medium and a hollow fiber cell culture apparatus.Significantly less time and space are required than in conventional methods, and the resulting adenovirus is collected in a much smaller volume, simplifying the purification steps.The protocol described is a reproducible, convenient, here biologically safe, and environmentally sound method for the production of adenoviral vectors for laboratory use and has the potential to scale-up the adenovirus production for clinical use.